First Report of <i>Pectobacterium versatile</i> Causing Blackleg of Potato in Serbia

HomePlant DiseaseVol. 106, No. 1First Report of Pectobacterium versatile Causing Blackleg Potato in Serbia PreviousNext DISEASE NOTE OPENOpen Access licenseFirst SerbiaSanja Markovi?, Sonja Mili? Komi?, Aleksandra Jeluši?, Renata Ili?i?, Ferenc Bagi, Slaviša Stankovi?, and Tatjana Popovi?Sanja Markovi?University Belgrade, Institute for Multidisciplinary Research, Belgrade 11030, SerbiaSearch more papers by this author, Komi?University Jeluši?University Ili?i?University Novi Sad, Faculty Agriculture, 21000 BagiUniversity Stankovi?https://orcid.org/0000-0003-0527-8741University Biology, 11000 Popovi?†Corresponding author: T. Popovi?; E-mail Address: [email protected]://orcid.org/0000-0003-0125-5905Institute Plant Protection Environment, author AffiliationsAuthors Affiliations Sanja Markovi?1 Komi?1 Jeluši?1 Ili?i?2 Bagi2 Stankovi?3 Popovi?4 † 1University 2University 3University 4Institute Published Online:8 Jan 2022https://doi.org/10.1094/PDIS-06-21-1128-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articlePotato blackleg is frequently observed the Ba?ka region Vojvodina province, one largest potato-growing areas Serbia. It usually occurs June July. In July 2020, symptoms (stem necrotic lesions, vascular discoloration, hollow stems, wilting whole plants) were noted on cultivar VR808 a 28-ha field Magli? village (GPS: 45.349325°N, 19.542768°E). Disease incidence was 20 25%. Isolations performed from 12 potato samples crystal violet pectate medium. Stem sections with brown lesions healthy tissue (?10 cm) surface sterilized 70% (w/v) EtOH rinsed sterile distilled water (SDW). Small pieces edges stem soaked phosphate-buffered saline min plated (Klement et al. 1990). Single colonies that formed pits after 48 h at 26°C restreaked onto nutrient agar, where creamy white smooth surfaces formed. Thirty isolates selected, DNA analyzed PCR using partial dnaX gene primers dnaXf/dnaXr Dickeya identification (S?awiak 2009). A characteristic band 535 bp amplified all sequence alignment showed two distinct groups isolates, which genetically uniform within each group. BLASTn established first group (19 isolates) had 99.79% identity NCBI-deposited strains 14A 3-2 Belarus (CP034276 CP024842) SCC1 Finland (CP021894). The remaining 11 sequences 100% carotovorum subsp. strain CFBP7081 Spain (MK516961). three Serbian P. (Pv1320, Pv1520, Pv1620) (Pcc2520) deposited GenBank as MW839571, MW805306, MW839572, MW805307, respectively. These results, indicating combined infection, signify Multilocus analysis proA (proAF1/proAR1) mdh (mdh2/mdh4) genes (Ma 2007; Moleleki 2013) grouped tested together NCBI. For both genes, revealed homology Finland. Three under MZ682623 25 MZ682620 22 mdh. According routine tests Pectobacteriaceae (Schaad 2001), microbiological traits identical (Portier 2019). Pathogenicity Pv1320, Pv1620 follows: (i) surface-sterilized tuber slices holes center filled 100 µl bacterial suspension (109 CFU/ml) test ability cause soft rot, (ii) 4-week-old plants third true leaf (?30 cm tall) inoculated injecting stems (107 108 5 above soil line. Controls treated SDW. Inoculated kept 25°C >70% RH. Each assay replicated twice. Soft rot appeared 24 inoculation. On initial manifested greasy elongated spots inoculation sites 2 days (DAI), subsequently extended along became necrotic. Whole-plant decay DAI; controls remained healthy. To complete Koch’s postulates, bacteria reisolated symptomatic confirmed sequencing dnaX. This report indicates caused diverse population. pathogen designated ‘Candidatus maceratum’ (Shirshikov 2018) renamed sp. nov. brasiliensis dominant province recently infection dianthicola (Markovi? 2021). Findings study are highly relevant, diversity pathogens likely due increasingly widespread distribution imported seed potatoes.The author(s) declare no conflict interest.References:Klement, Z., 1990. Methods Phytobacteriology. Akademiai Kiado, Budapest, Hungary. Google ScholarMa, B., 2007. Phytopathology 97:1150. https://doi.org/10.1094/PHYTO-97-9-1150 Link, ISI, ScholarMarkovi?, S., 2021. Dis. 105:1080. https://doi.org/10.1094/PDIS-05-20-1076-RE ScholarMoleleki, L. N., 2013. Eur. J. Pathol. 135:279. https://doi.org/10.1007/s10658-012-0084-4 Crossref, ScholarPortier, P., 2019. Int. Syst. Evol. Microbiol. 69:3207. https://doi.org/10.1099/ijsem.0.003611 ScholarSchaad, N. W., 2001. Laboratory Guide Identification Pathogenic Bacteria, 3rd Ed. APS Press, St. Paul, MN. ScholarShirshikov, F. V., 2018. Genome Announc. 6:e00260-18. https://doi.org/10.1128/genomeA.00260-18 ScholarS?awiak, M., 2009. 125:245. https://doi.org/10.1007/s10658-009-9479-2 ScholarFunding: work supported Ministry Education, Science Technological Development Republic Serbia, contract numbers 451-03-9/2021-14/200010, 451-03-9/2021-14/200053, 451-03-9/2021-14/200117.The interest.DetailsFiguresLiterature CitedRelated Vol. 1 January 2022SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptoms Macrophomina phaseolina melon (R. Cohen al.). Photo credit: R. Cohen. Jute plant infected papaya ring spot virus (PRSV) (sample MG16-004) (C. Biswas V. Ramesh Babu. Metrics Downloaded 783 times Article History Issue Date: 7 Feb 2022Published: 8 2022First Look: 19 Aug 2021Accepted: 17 2021 Page: 312 Information© 2022 American Phytopathological SocietyFundingMinistry DevelopmentGrant/Award Number: 451-03-9/2021-14/200010Grant/Award 451-03-9/2021-14/200053Grant/Award 451-03-9/2021-14/200117Keywordspotatoblacklegpathogen detectionprokaryotesThe interest.PDF downloadCited ByVolatile Organic Compounds Physiological Parameters Markers (Solanum tuberosum L.) Infection Phytopathogens9 | Molecules, 27, 12Encapsulated Clove Bud Essential Oil: New Perspective an Eco-Friendly Biopesticide27 February 12, 3